Western blot was performed using Anti-RICTOR Polyclonal Antibody (Product # MA5-15006) and a 192 kDa band corresponding to RICTOR was observed across cell lines. Whole cell extracts (40 µg lysate) of SW480 (Lane 1), BeWo (Lane 2), HeLa (Lane 3), MOLT4 (Lane 4), HCT 116 serum starved overnight (Lane 5), HCT116 serum starved overnight and treated with EGF (20 ng/mL for 45 mins) (Lane 6), HCT116 serum starved overnight, treated with Curcumin 25 µM for 2 hrs and then treated with EGF (20 ng/mL for 45 mins) (Lane 7), A549 (Lane 8), A549 treated with MG132 5 µM for 3 hrs (Lane 9) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (*1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).